Journal: Iranian Biomedical Journal

Article Title: The Lentiviral Vector Pseudotyped by Modified Rabies Glycoprotein Does Not Cause Reactive Gliosis and Neurodegeneration in Rat Hippocampus

doi: 10.29252/.23.5.324

Figure Lengend Snippet: Schematic representation and frequency of green fluorescent protein (GFP)-expressed neurons in CA1 and CA3 of the rat hippocampus one week after injection of the lentiviral vector into CA1. Upper part, left: a coronal section of the rat brain stained by hematoxylin and eosin. Upper part, LV-RG: double-staining of the hippocampal cells of the vector-injected rats by anti-GFP and anti-NeuN monoclonal antibodies. Upper part, control: staining of the hippocampal cells of the saline-injected rats by anti-neuronal Nuclear protein (NeuN) monoclonal antibody. Lower part: frequency of neurons in CA1 and CA3 regions of rat hippocampus, one week after delivery of the lentivector or saline into CA1. No significant difference was found in the frequency of NeuN-positive cells between control and the vector-injected groups in both regions. LV-RG: the HIV-1-based lentiviral vector pseudotyped by the modified rabies glycoprotein, FUG-B2. Scale bar: 100 µm. Magnification: 20 , 400 × 350 µm 2

Article Snippet: Materials The monoclonal rabbit anti-green fluorescent protein (GFP), mouse anti-neuronal nuclear protein (NeuN), mouse monoclonal anti-glial fibrillary acidic protein (GFAP), and secondary Texas Red-X conjugated goat anti-mouse IgG antibodies were purchased from Merck, Millipore (USA).

Techniques: Injection, Plasmid Preparation, Staining, Double Staining, Bioprocessing, Control, Saline, Modification